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  • Featured Product
  • KD/KO Validated

ALDH1A1 Polyclonal antibody

ALDH1A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15910-1-AP

Synonyms

ALDH1A 1, ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ALDH1A1 Polyclonal antibody (15910-1-AP)

    WB analysis using 15910-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDH1A1 Polyclonal antibody (15910-1-AP)

    WB analysis using 15910-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ALDH1A1 Polyclonal antibody (15910-1-AP)

    WB analysis using 15910-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15910-1-AP (ALDH1A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IP experiment of mouse liver using 15910-1-AP

    IP result of anti-ALDH1A1 (IP:15910-1-AP, 4ug; Detection:15910-1-AP 1:20000) with mouse liver tissue lysate 2200 ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IHC staining of mouse testis using 15910-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IHC staining of mouse testis using 15910-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IHC staining of human colon using 15910-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IHC staining of human liver using 15910-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IHC staining of human liver using 15910-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IF Staining of mouse colon using 15910-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IF Staining of mouse colon using 15910-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using 15910-1-AP (ALDH1A1 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IF Staining of mouse testis using 15910-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 15910-1-AP (ALDH1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using ALDH1A1 Polyclonal antibody (15910-1-AP)

    IF Staining of A549 using 15910-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using ALDH1A1 antibody (15910-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using ALDH1A1 Polyclonal antibody (15910-1-AP)

    FC experiment of HepG2 using 15910-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human ALDH1A1 (15910-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inK-562 cells, mouse liver tissue, rat liver tissue, mouse brain tissue, mouse kidney tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inmouse testis tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue, mouse testis tissue
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15910-1-AP targets ALDH1A1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ALDH1A1 fusion protein Ag8665
Full Name aldehyde dehydrogenase 1 family, member A1
Calculated Molecular Weight 501 aa, 55 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC001505
Gene Symbol ALDH1A1
Gene ID (NCBI) 216
RRIDAB_2305276
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001). This antibody can also recognize some other membranes of the aldehyde dehydrogenase family.

Protocols

Product Specific Protocols
WB protocol for ALDH1A1 antibody 15910-1-APDownload protocol
IHC protocol for ALDH1A1 antibody 15910-1-APDownload protocol
IF protocol for ALDH1A1 antibody 15910-1-APDownload protocol
IP protocol for ALDH1A1 antibody 15910-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Res

Excessive UBE3A dosage impairs retinoic acid signaling and synaptic plasticity in autism spectrum disorders.

Authors - Xingxing Xu
View Article
mouseWB

Mol Cell

Maternal Aldehyde Elimination during Pregnancy Preserves the Fetal Genome.

Authors - Nina Oberbeck
View Article
humanWB,IHC,IF

Int J Oral Sci

LIMP-2 enhances cancer stem-like cell properties by promoting autophagy-induced GSK3β degradation in head and neck squamous cell carcinoma

Authors - Yuantong Liu
View Article
humanWB,IHC

Nat Commun

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Authors - Rongxuan Zhu
View Article
human,mouseWB,IHC,IF

J Pineal Res

Melatonin inhibits the stemness of head and neck squamous cell carcinoma by modulating HA synthesis via the FOSL1/HAS3 axis

Authors - Xinyue Luo
View Article
humanIF

EMBO J

RASSF1C oncogene elicits amoeboid invasion, cancer stemness, and extracellular vesicle release via a SRC/Rho axis.

Authors - Maria Laura Tognoli
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Shinford Z. (Verified Customer) (01-31-2024)

The antibody performs exceptionally well; the band on the membrane is remarkably distinct.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cancer cell lines
ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in Cancer cell lines (Cat no:15910-1-AP)
FH

Matthew (Verified Customer) (08-07-2023)

Antibody worked perfectly with overnight incubation at 4C. Diluted 1:1000 in TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse mammary epithelial cell
FH

Udesh (Verified Customer) (02-24-2023)

Worked well at 1:1000 in 5% BSA O/N at 4 degrees

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U2OS Osteosarcoma cells
ALDH1A1 Antibody Western Blot validation (1:1000 dilution) in U2OS Osteosarcoma cells (Cat no:15910-1-AP)
FH

Susmita (Verified Customer) (09-17-2020)

Antibody is working good for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000 WB;
FH

Mario Armando (Verified Customer) (02-18-2020)

I used this ALDH1A1 antibody on different human and mouse tissues including adipose tissue, heart and skeletal muscle. It worked wonderfully even when using paraffin embedded sections in both human and mouse.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: OCT embedded frozen section of Human Adipose tissue
ALDH1A1 Antibody Immunofluorescence, validation (1:100 dilution) in OCT embedded frozen section of Human Adipose tissue (Cat no:15910-1-AP)
FH

Joleen (Verified Customer) (06-07-2019)

Prominent band correspond to ALDH1A1. Works well in western blot.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1k
  • Cell Tissue Type: MDCK GII
ALDH1A1 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:15910-1-AP)