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  • Featured Product
  • KD/KO Validated

Albumin Polyclonal antibody

Albumin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16475-1-AP

Synonyms

ALB, Serum albumin, HSA

Filter:
  • Western Blot (WB) analysis of HepG2 cells using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis of HepG2 using 16475-1-AP

    WB result of Albumin antibody (16475-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Albumin transfected HepG2 cells.

  • Western Blot (WB) analysis of human plasma using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis of human plasma using 16475-1-AP

    human blood were subjected to SDS PAGE followed by western blot with 16475-1-AP (ALB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis using 16475-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse serum using Albumin Polyclonal antibody (16475-1-AP)

    WB analysis of mouse serum using 16475-1-AP

    mouse serum were subjected to SDS PAGE followed by western blot with 16475-1-AP (Albumin Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using Albumin Polyclonal antibody (16475-1-AP)

    IP experiment of human plasma using 16475-1-AP

    IP result of anti-Albumin (IP:16475-1-AP, 4ug; Detection:16475-1-AP 1:10000) with human plasma lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of human liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of human liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of human colon using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16475-1-AP (Albumin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of mouse liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IHC staining of mouse liver using 16475-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16475-1-AP (Albumin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of mouse liver using 16475-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of mouse liver using 16475-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse liver tissue using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of mouse liver using 16475-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using Albumin antibody (16475-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of L02 cells using Albumin Polyclonal antibody (16475-1-AP)

    IF Staining of L02 using 16475-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using Albumin antibody (16475-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

"Albumin Antibodies" Comparison

View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.

Tested Applications

Positive WB detected inhuman blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver tissue
Positive IP detected inhuman plasma tissue
Positive IHC detected inhuman liver tissue, mouse liver tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse liver tissue
Positive IF/ICC detected inL02 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16475-1-AP targets Albumin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Albumin fusion protein Ag9569
Full Name albumin
Calculated Molecular Weight 609 aa, 69 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC034023
Gene Symbol Albumin
Gene ID (NCBI) 213
RRIDAB_2242567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)

Protocols

Product Specific Protocols
WB protocol for Albumin antibody 16475-1-APDownload protocol
IHC protocol for Albumin antibody 16475-1-APDownload protocol
IF protocol for Albumin antibody 16475-1-APDownload protocol
IP protocol for Albumin antibody 16475-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Science

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Authors - Yong-Hyun Han
View Article
mouseIF

Signal Transduct Target Ther

MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration

Authors - Jinpeng Du
View Article
human, mouseWB,IF

Nat Immunol

Exosomes mediate the cell-to-cell transmission of IFN-α-induced antiviral activity.

Authors - Li Jianhua J
View Article
humanIHC

Immunity

The zinc finger protein Miz1 suppresses liver tumorigenesis by restricting hepatocyte-driven macrophage activation and inflammation.

Authors - Wenjie Zhang
View Article
mouseWB

Nat Cell Biol

Manganese regulation of COPII condensation controls circulating lipid homeostasis

Authors - Xiao Wang
View Article
humanWB

Cell Res

In vivo self-assembled small RNAs as a new generation of RNAi therapeutics.

Authors - Zheng Fu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Kamal (Verified Customer) (01-26-2024)

For western blot analysis, antibody worked well at dilution of 1:25000 in 1 X TBST. For immunofluorescence, antibody worked well at dilution of 1:200 in 1 X PBS.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: Mouse bone marrow
FH

Kamal (Verified Customer) (01-26-2024)

Liver tissues were well stained in provided dilution with 1X PBS.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse liver tissue