AKT Polyclonal antibody
AKT Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, Mouse and More (2)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55230-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells |
| Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
Product Information
55230-1-AP targets AKT in WB, IHC, ELISA applications and shows reactivity with human, Mouse samples.
| Tested Reactivity | human, Mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-62 kDa |
| GenBank Accession Number | NM_001014431 |
| Gene Symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| RRID | AB_2881292 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKT1, also named as PKB and RAC, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and RAC subfamily. It plays a role as a key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. AKT1 promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase. It plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. AKT1 mediates the antiapoptotic effects of IGF-I. (PMID:16139227)This antibody is specific to AKT1. It has no cross reaction to AKT2 and AKT3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKT antibody 55230-1-AP | Download protocol |
| IHC protocol for AKT antibody 55230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Med Rep Matrine inhibits the invasive and migratory properties of human hepatocellular carcinoma by regulating epithelial‑mesenchymal transition. | ||
J Immunol The Intracellular Interaction of Porcine β-Defensin 2 with VASH1 Alleviates Inflammation via Akt Signaling Pathway. | ||
Int J Mol Sci Extracellular Calcium-Induced Calcium Transient Regulating the Proliferation of Osteoblasts through Glycolysis Metabolism Pathways | ||
J Ethnopharmacol Shenqu xiaoshi oral solution enhances digestive function and stabilizes the gastrointestinal microbiota of juvenile rats with infantile anorexia | ||
Acta Biochim Biophys Sin (Shanghai) Multiple circRNAs regulated by QKI5 conjointly spongemiR-214-3p to antagonize bisphenol A-inducedspermatocyte toxicity |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Tanusree (Verified Customer) (12-18-2019) | Product worked well in WB at 1:500 dilution and 1:100 in IF
|
FH Tanusree (Verified Customer) (08-14-2019) | The antibody works good in mouse brain tissue in western blotting.
|
