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- KD/KO Validated
AKT1/3 Monoclonal antibody
AKT1/3 Monoclonal Antibody for WB, IP, IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
CloneNo.
3G5E11
Cat no : 60203-1-Ig
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, rat liver tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 54 publications below |
| IF | See 1 publications below |
Product Information
The immunogen of 60203-1-Ig is AKT1/3 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, huamn |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | AKT1/3 fusion protein Ag16695 |
| Full Name | v-akt murine thymoma viral oncogene homolog 1 |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 56-62 kDa |
| GenBank Accession Number | BC000479 |
| Gene Symbol | AKT1 |
| Gene ID (NCBI) | 207 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The serine-threonine protein kinase AKT is catalytically inactive in serum-starved primary and immortalized fibroblasts. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT, which then phosphorylates and inactivates components of the apoptotic machinery.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
| IHC protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
| IF protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
| IP protocol for AKT1/3 antibody 60203-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Circular RNA circATP9A promotes non-small cell lung cancer progression by interacting with HuR and by promoting extracellular vesicles-mediated macrophage M2 polarization | ||
Sci Total Environ Effects of triclosan exposure on stem cells from human exfoliated deciduous teeth (SHED) fate | ||
Autophagy BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. | ||
Cancer Lett Endogenous production of C-C motif chemokine ligand 2 by nasopharyngeal carcinoma cells drives radioresistance-associated metastasis. |
