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AKR1CL2 Polyclonal antibody

AKR1CL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20525-1-AP

Synonyms

Aldo-keto reductase family 1 member C-like protein 2, AKRDC1, AKR1E2, AKR1CL 2, AF reductase

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of mouse testis using 20525-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW264.7 using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of RAW264.7 using 20525-1-AP

    RAW264.7 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HT-1080 cells using AKR1CL2 Polyclonal antibody (20525-1-AP)

    WB analysis of HT-1080 using 20525-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20525-1-AP (AKR1CL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AKR1CL2 Polyclonal antibody (20525-1-AP)

    IF Staining of HeLa using 20525-1-AP

    Immunofluorescent analysis of Hela cells, using AKR1CL2 antibody 20525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse testis tissue, HT-1080 cells
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20525-1-AP targets AKR1CL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1CL2 fusion protein Ag14381
Full Name aldo-keto reductase family 1, member C-like 2
Calculated Molecular Weight 320 aa, 37 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC002862
Gene Symbol AKR1CL2
Gene ID (NCBI) 83592
RRIDAB_10694285
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKR1CL2 antibody 20525-1-APDownload protocol
IF protocol for AKR1CL2 antibody 20525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols