AKR1C3 Polyclonal antibody

AKR1C3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11194-1-AP

Synonyms

17 beta HSD 5, 3 alpha HSD type 2, 3 alpha HSD type II, brain, AKR1C3, DD 3, DD3, DDX, Dihydrodiol dehydrogenase 3, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, Indanol dehydrogenase, KIAA0119, PGFS, Prostaglandin F synthase



Tested Applications

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11194-1-AP targets AKR1C3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1C3 fusion protein Ag1674
Full Name aldo-keto reductase family 1, member C3 (3-alpha hydroxysteroid dehydrogenase, type II)
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC019230
Gene Symbol AKR1C3
Gene ID (NCBI) 8644
RRIDAB_2224414
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKR1C3(Aldo-keto reductase family 1 member C3) is also named as DDH1, HSD17B5, KIAA0119, PGFS and belongs to AKR1C family. .In humans, at least four AKR1C isoforms exist: AKR1C1, AKR1C2, AKR1C3, AKR1C4 and AKR1C3 shares >86% sequence identity with these three highly related human AKRs(PMID:18574251). It catalyzes the conversion of aldehydes and ketones to alcohols and androgen, estrogen, PG, xenobiotics metabolism. The rat kidney possesses a dimeric form of 75 kDa(PMID:18574251).

Protocols

Product Specific Protocols
WB protocol for AKR1C3 antibody 11194-1-APDownload protocol
IHC protocol for AKR1C3 antibody 11194-1-APDownload protocol
IP protocol for AKR1C3 antibody 11194-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cancer Ther

AKR1C3 promotes AR-V7 protein stabilization and confers resistance to AR-targeted therapies in advanced prostate cancer.

Authors - Chengfei Liu
humanWB,IHC

World J Gastroenterol

Prostaglandin F2α synthase promotes oxaliplatin resistance in colorectal cancer through prostaglandin F2α-dependent and F2α-independent mechanism

Authors - Yi-Jun Wang
humanWB

Int J Mol Med

15-Deoxy-Δ(12,14)-prostaglandin J(2) induces growth inhibition, cell cycle arrest and apoptosis in human endometrial cancer cell lines.

Authors - Li Haili H
mouse,ratWB

Neurosci Lett

Mangiferin alleviates 6-OHDA-induced Parkinson's disease by inhibiting AKR1C3 to activate Wnt signaling pathway

Authors - Wanran Huang
humanIHC

J Clin Med

A New Histology-Based Prognostic Index for Aggressive T-Cell lymphoma: Preliminary Results of the "TCL Urayasu Classification"

Authors - Hideaki Nitta
humanWB

Eur J Med Chem

Development of highly potent and specific AKR1C3 inhibitors to restore the chemosensitivity of drug-resistant breast cancer

Authors - Yang Liu