AKR1B10 Polyclonal antibody
AKR1B10 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 18252-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, mouse liver tissue, HepG2 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
18252-1-AP targets AKR1B10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKR1B10 fusion protein Ag13042 |
| Full Name | aldo-keto reductase family 1, member B10 (aldose reductase) |
| Calculated Molecular Weight | 316 aa, 36 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC008837 |
| Gene Symbol | AKR1B10 |
| Gene ID (NCBI) | 57016 |
| RRID | AB_2878524 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1B10 antibody 18252-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Pharmacol Exp Ther Mechanistic Analysis of an Extracellular Signal-Regulated Kinase 2-Interacting Compound that Inhibits Mutant BRAF-Expressing Melanoma Cells by Inducing Oxidative Stress. | ||
J Ethnopharmacol Berberine directly targets AKR1B10 protein to modulate lipid and glucose metabolism disorders in NAFLD
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Shinford (Verified Customer) (10-26-2023) | In tissues, we detected bands at different positions, but in cancer cell lines, the bands appeared notably clearer. The optimal concentration was determined to be higher, specifically at 1:1000.
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