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  • Featured Product
  • KD/KO Validated

AKR1A1 Polyclonal antibody

AKR1A1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15054-1-AP

Synonyms

EC:1.1.1.19, ALR, ALDR1, Aldo-keto reductase family 1 member A1, Alcohol dehydrogenase [NADP(+)]

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AKR1A1 Polyclonal antibody (15054-1-AP)

    WB analysis using 15054-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15054-1-AP (AKR1A1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AKR1A1 Polyclonal antibody (15054-1-AP)

    IP experiment of HeLa using 15054-1-AP

    IP result of anti-AKR1A1 (IP:15054-1-AP, 4ug; Detection:15054-1-AP 1:500) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AKR1A1 Polyclonal antibody (15054-1-AP)

    IF Staining of HeLa using 15054-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15054-1-AP (AKR1A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, L02 cells, mouse lung tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15054-1-AP targets AKR1A1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKR1A1 fusion protein Ag7037
Full Name aldo-keto reductase family 1, member A1 (aldehyde reductase)
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000670
Gene Symbol AKR1A1
Gene ID (NCBI) 10327
RRIDAB_2224138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKR1A1 antibody 15054-1-APDownload protocol
IF protocol for AKR1A1 antibody 15054-1-APDownload protocol
IP protocol for AKR1A1 antibody 15054-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC,IP

Nature

Metabolic reprogramming by the S-nitroso-CoA reductase system protects against kidney injury.

Authors - Hua-Lin Zhou
  • KO Validated
View Article
mouseWB

J Med Chem

Identification of a Selective SCoR2 Inhibitor That Protects Against Acute Kidney Injury

Authors - Hua-Lin Zhou
View Article
mouseWB

J Biol Chem

AKR1A1 is a novel mammalian S-nitroso-glutathione reductase.

Authors - Colin T Stomberski
  • KO Validated
View Article