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AKNA Polyclonal antibody

AKNA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 23785-1-AP

Synonyms

AKNA, AT hook transcription factor, KIAA1968, Microtubule organization protein AKNA, RP11 82I1.4

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using AKNA Polyclonal antibody (23785-1-AP)

    WB analysis of K-562 using 23785-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 23785-1-AP (AKNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23785-1-AP is AKNA Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKNA fusion protein Ag20662
Full Name AT-hook transcription factor
Calculated Molecular Weight 155 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC055285
Gene Symbol AKNA
Gene ID (NCBI) 80709
RRIDAB_2879324
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKNA also named as AT hook transcription factor is a 1439 amino-acids protein. AKNA, encodes a lymphoid-specific AT-hook transcription factor that is highly expressed by B and T lymphocytes. It coordinately regulates expression of the CD40 receptor and its ligand (CD154), two molecules that are critical for antigen-dependent B-cell development. AKNA expresses at least nine distinct transcripts, some of which are expressed in a tissue-specific manner. These transcripts are products of alternative splicing, alternative promoter usage, and alternative polyadenylation. The alternative AKNA transcripts are predicted to encode overlapping protein isoforms, some of which (p70 and p100) (PMID:15869410)

Protocols

Product Specific Protocols
WB protocol for AKNA antibody 23785-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biomed Res Int

AKNA Is a Potential Prognostic Biomarker in Gastric Cancer and Function as a Tumor Suppressor by Modulating EMT-Related Pathways.

Authors - Gang Wang
View Article