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AKD1 Polyclonal antibody

AKD1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23042-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using AKD1 Polyclonal antibody (23042-1-AP)

    WB analysis of HEK-293 using 23042-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23042-1-AP (AKD1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AKD1 Polyclonal antibody (23042-1-AP)

    IHC staining of human kidney using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AKD1 Polyclonal antibody (23042-1-AP)

    IHC staining of human testis using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AKD1 Polyclonal antibody (23042-1-AP)

    IHC staining of human testis using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AKD1 Polyclonal antibody (23042-1-AP)

    IHC staining of human kidney using 23042-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23042-1-AP (AKD1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using AKD1 Polyclonal antibody (23042-1-AP)

    IF Staining of HEK-293 using 23042-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using AKD1 antibody (23042-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23042-1-AP targets AKD1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKD1 fusion protein Ag19038
Full Name adenylate kinase domain containing 1
Calculated Molecular Weight 1911 aa, 221 kDa
Observed Molecular Weight 40-49 kDa
GenBank Accession NumberBC153055
Gene Symbol AKD1
Gene ID (NCBI) 401271
RRIDAB_2879201
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AKD1 antibody 23042-1-APDownload protocol
IHC protocol for AKD1 antibody 23042-1-APDownload protocol
IF protocol for AKD1 antibody 23042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols