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  • Featured Product
  • KD/KO Validated

AKAP8L Polyclonal antibody

AKAP8L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10103-1-AP

Synonyms

HA95, A-kinase anchor protein 8-like, AKAP95, AKAP8-like protein, AKAP8 like protein

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using AKAP8L Polyclonal antibody (10103-1-AP)

    WB analysis of PC-3 using 10103-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10103-1-AP (AKAP8L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using AKAP8L Polyclonal antibody (10103-1-AP)

    IF Staining of HEK-293 using 10103-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10103-1-AP (AKAP8L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inPC-3 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10103-1-AP targets AKAP8L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP8L fusion protein Ag0145
Full Name A kinase (PRKA) anchor protein 8-like
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000713
Gene Symbol AKAP8L
Gene ID (NCBI) 26993
RRIDAB_2258197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP8L, also named as NAKAP, or NAKAP95, is a 646 amino acid protein, which contains two C2H2 AKAP95-type zinc fingers and belongs to the AKAP95 family. AKAP8L localizes in the nucleus matrix and is expressed in the brain cortex. AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. AKAP8L may be involved in nuclear envelope breakdown and chromatin condensation. It also may regulate the initiation phase of DNA replication when associated with TMPO-beta.

Protocols

Product Specific Protocols
WB protocol for AKAP8L antibody 10103-1-APDownload protocol
IF protocol for AKAP8L antibody 10103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Retrovirology

The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3 to HIV-1 RNA.

Authors - Li Xing
  • KD Validated
View Article
humanWB

RNA Biol

hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression.

Authors - Tanzina Tanu
  • KD Validated
View Article