AKAP4 Polyclonal antibody
AKAP4 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 24986-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 2 publications below |
Product Information
The immunogen of 24986-1-AP is AKAP4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | AKAP4 fusion protein Ag21762 |
| Full Name | A kinase (PRKA) anchor protein 4 |
| Calculated Molecular Weight | 854 aa, 94 kDa |
| Observed Molecular Weight | 82 kDa |
| GenBank Accession Number | BC126252 |
| Gene Symbol | AKAP4 |
| Gene ID (NCBI) | 8852 |
| RRID | AB_2876859 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKAP4, also named as A-kinase anchor protein 82 kDa, is a 854 amino acid protein, which belongs to the AKAP110 family. AKAP4 localizes to the principle piece of the sperm flagellum and is only expressed in round spermatids. AKAP4 is a major structural component of sperm fibrous sheath and plays a role in sperm motility.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for AKAP4 antibody 24986-1-AP | Download protocol |
| IHC protocol for AKAP4 antibody 24986-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness. | ||
Andrologia Successful outcomes of intracytoplasmic sperm injection-embryo transfer using ejaculated spermatozoa from two Chinese asthenoteratozoospermic brothers with a compound heterozygous FSIP2 mutation. | ||
Life Sci Alliance Genetic mutation of Cep76 results in male infertility due to abnormal sperm tail composition | ||
Sci China Life Sci Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes | ||
