AKAP3 Polyclonal antibody
AKAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 13907-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse testis tissue, rat testis tissue |
Positive IHC detected in | rat testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse testis tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 12 publications below |
IHC | See 2 publications below |
IF | See 9 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
13907-1-AP targets AKAP3 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AKAP3 fusion protein Ag4885 |
Full Name | A kinase (PRKA) anchor protein 3 |
Calculated Molecular Weight | 95 kDa |
Observed Molecular Weight | 100 kDa |
GenBank Accession Number | BC047535 |
Gene Symbol | AKAP3 |
Gene ID (NCBI) | 10566 |
RRID | AB_2273887 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.
Protocols
Product Specific Protocols | |
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WB protocol for AKAP3 antibody 13907-1-AP | Download protocol |
IHC protocol for AKAP3 antibody 13907-1-AP | Download protocol |
IF protocol for AKAP3 antibody 13907-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness. | ||
Proc Natl Acad Sci U S A SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. | ||
Development The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development | ||
J Genet Genomics Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice | ||
Development The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development. | ||
Front Cell Dev Biol Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. |