AKAP3 Polyclonal antibody

AKAP3 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13907-1-AP

Synonyms

A-kinase anchor protein 110 kDa, AKAP-3, AKAP110, AKAP 3, AKAP 110



Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inrat testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13907-1-AP targets AKAP3 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP3 fusion protein Ag4885
Full Name A kinase (PRKA) anchor protein 3
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
RRIDAB_2273887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.

Protocols

Product Specific Protocols
WB protocol for AKAP3 antibody 13907-1-APDownload protocol
IHC protocol for AKAP3 antibody 13907-1-APDownload protocol
IF protocol for AKAP3 antibody 13907-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proc Natl Acad Sci U S A

FSIP1 binds HER2 directly to regulate breast cancer growth and invasiveness.

Authors - Tong Liu
mouseWB

Proc Natl Acad Sci U S A

SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice.

Authors - Haruhiko Miyata
mouseIF

Development

The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development

Authors - Anja Beckers
mouseWB,IF

J Genet Genomics

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

Authors - Yaling Wang
mouseIF

Development

The FOXJ1 target Cfap206 is required for sperm motility, mucociliary clearance of the airways and brain development.

Authors - Anja Beckers
pigWB

Front Cell Dev Biol

Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.

Authors - Yongjie Xu