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AKAP1 Polyclonal antibody

AKAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15618-1-AP

Synonyms

D-AKAP-1, DAKAP1, A-kinase anchor protein 149 kDa, A-kinase anchor protein 1, mitochondrial, AKAP 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using AKAP1 Polyclonal antibody (15618-1-AP)

    WB analysis of HeLa using 15618-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using AKAP1 Polyclonal antibody (15618-1-AP)

    WB analysis of PC-3 using 15618-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    WB analysis of human liver using 15618-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AKAP1 Polyclonal antibody (15618-1-AP)

    WB analysis of MCF-7 using 15618-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 15618-1-AP (AKAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of PC-3 cells using AKAP1 Polyclonal antibody (15618-1-AP)

    IP experiment of PC-3 using 15618-1-AP

    IP result of anti-AKAP1 (IP:15618-1-AP, 4ug; Detection:15618-1-AP 1:2000) with PC-3 cells lysate 1800ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human lung cancer using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human lung cancer using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15618-1-AP (AKAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human colon cancer using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human colon cancer using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human small intestine using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human small intestine using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 15618-1-AP (AKAP1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human prostate cancer using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AKAP1 Polyclonal antibody (15618-1-AP)

    IHC staining of human prostate cancer using 15618-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15618-1-AP (AKAP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AKAP1 Polyclonal antibody (15618-1-AP)

    IF Staining of MCF-7 using 15618-1-AP

    Immunofluorescent analysis of MCF-7 cells, using AKAP1 antibody 15618-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHeLa cells, human liver tissue, MCF-7 cells, PC-3 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman lung cancer tissue, human prostate cancer tissue, human colon cancer tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15618-1-AP targets AKAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AKAP1 fusion protein Ag8037
Full Name A kinase (PRKA) anchor protein 1
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC000729
Gene Symbol AKAP1
Gene ID (NCBI) 8165
RRIDAB_2225484
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AKAP1 (also termed AKAP149) is a human 149 kDa anchoring protein localized in mitochondria and the endoplasmic reticulum/nuclear envelope (ER-NE) network. It Binds to type I and II regulatory subunits of protein kinase A and anchors them to the cytoplasmic face of the mitochondrial outer membrane. This protein is speculated to be involved in the cAMP-dependent signal transduction pathway and in directing RNA to a specific cellular compartment.

Protocols

Product Specific Protocols
WB protocol for AKAP1 antibody 15618-1-APDownload protocol
IHC protocol for AKAP1 antibody 15618-1-APDownload protocol
IF protocol for AKAP1 antibody 15618-1-APDownload protocol
IP protocol for AKAP1 antibody 15618-1-APDownload protocol
FC protocol for AKAP1 antibody 15618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.

Authors - Wenxian Wu
View Article
humanWB,IP,IF

Mol Endocrinol

Short RNA molecules with high binding affinity to the KH motif of A-kinase anchoring protein 1 (AKAP1): implications for the regulation of steroidogenesis.

Authors - Grozdanov Petar N PN
View Article