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AK5 Polyclonal antibody

AK5 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 12510-2-AP

Synonyms

KAD5, EC:2.7.4.3, ATP-AMP transphosphorylase 5, AK 5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK5 Polyclonal antibody (12510-2-AP)

    WB analysis using 12510-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12510-2-AP (AK5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse cerebellum tissue using AK5 Polyclonal antibody (12510-2-AP)

    WB analysis of mouse cerebellum using 12510-2-AP

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12510-2-AP (AK5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AK5 Polyclonal antibody (12510-2-AP)

    IHC staining of mouse brain using 12510-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AK5 Polyclonal antibody (12510-2-AP)

    IHC staining of mouse brain using 12510-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AK5 Polyclonal antibody (12510-2-AP)

    IHC staining of mouse brain using 12510-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using AK5 Polyclonal antibody (12510-2-AP)

    IHC staining of mouse brain using 12510-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12510-2-AP (AK5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AK5 Polyclonal antibody (12510-2-AP)

    IF Staining of mouse brain using 12510-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12510-2-AP (AK5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using AK5 Polyclonal antibody (12510-2-AP)

    IF Staining of mouse brain using 12510-2-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 12510-2-AP (AK5 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, mouse cerebellum tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12510-2-AP targets AK5 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AK5 fusion protein Ag3203
Full Name adenylate kinase 5
Calculated Molecular Weight 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC012467
Gene Symbol AK5
Gene ID (NCBI) 26289
RRIDAB_2305376
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AK5 antibody 12510-2-APDownload protocol
IHC protocol for AK5 antibody 12510-2-APDownload protocol
IF protocol for AK5 antibody 12510-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human, ratWB,IF

J Neurol Sci

Down-regulation of adenylate kinase 5 in temporal lobe epilepsy patients and rat model.

Authors - Yujie Lai
View Article