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  • Featured Product
  • KD/KO Validated

AK3L1 Polyclonal antibody

AK3L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13206-1-AP

Synonyms

AK4, AK3L 1, AK3, AK 4, Adenylate kinase 4, mitochondrial

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using AK3L1 Polyclonal antibody (13206-1-AP)

    WB analysis of MCF-7 using 13206-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using AK3L1 Polyclonal antibody (13206-1-AP)

    WB analysis of human liver using 13206-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13206-1-AP (AK3L1 antibody) at dilution of 1:1800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using AK3L1 Polyclonal antibody (13206-1-AP)

    IP experiment of MCF-7 using 13206-1-AP

    IP result of anti-AK3L1 (IP:13206-1-AP, 4ug; Detection:13206-1-AP 1:300) with MCF-7 cells lysate 3200 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AK3L1 Polyclonal antibody (13206-1-AP)

    IHC staining of human breast cancer using 13206-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using AK3L1 Polyclonal antibody (13206-1-AP)

    IHC staining of human breast cancer using 13206-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13206-1-AP (AK3L1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using AK3L1 Polyclonal antibody (13206-1-AP)

    IF Staining of MCF-7 using 13206-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 13206-1-AP (AK3L1 antibody) at dilution of 1:100 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inMCF7 cells, human liver tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3600
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13206-1-AP targets AK3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AK3L1 fusion protein Ag3966
Full Name adenylate kinase 3-like 1
Calculated Molecular Weight 223 aa, 25 kDa
Observed Molecular Weight 30 kDa
GenBank Accession NumberBC040224
Gene Symbol AK3L1
Gene ID (NCBI) 205
RRIDAB_2225456
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AK3L1(Adenylate kinase 3-like) is also named as AK4, AK3L1 and belongs to the adenylate kinase family. It catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. It is important for maintenance of homeostasis of the adenine and guanine nucleotide pools. Expression is highest in kidney and heart, moderate in liver, weak in brain, and barely detectable in placenta and lung by northern blot(PMID:11485571). This antibody may also recognize AK3 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for AK3L1 antibody 13206-1-APDownload protocol
IHC protocol for AK3L1 antibody 13206-1-APDownload protocol
IF protocol for AK3L1 antibody 13206-1-APDownload protocol
IP protocol for AK3L1 antibody 13206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Cell Int

The miR-199a-3p regulates the radioresistance of esophageal cancer cells via targeting the AK4 gene.

Authors - Chunbao Zang
  • KD Validated
View Article
ratWB,IHC

Exp Brain Res

MicroRNA-124 conducts neuroprotective effect via inhibiting AK4/ATF3 after subarachnoid hemorrhage

Authors - Wei Jiang
View Article