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  • Featured Product
  • KD/KO Validated

AK2 Monoclonal antibody

AK2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat no : 66127-1-Ig

Synonyms

ATP:AMP phosphotransferase, ADK2, Adenylate monophosphate kinase, Adenylate kinase 2, mitochondrial, N-terminally processed, adenylate kinase 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AK2 Monoclonal antibody (66127-1-Ig)

    WB analysis using 66127-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Western Blot (WB) analysis of HeLa cells using AK2 Monoclonal antibody (66127-1-Ig)

    WB analysis of HeLa using 66127-1-Ig

    WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells.

  • Western Blot (WB) analysis of HEK-293 cells using AK2 Monoclonal antibody (66127-1-Ig)

    WB analysis of HEK-293 using 66127-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human liver using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of rat heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of rat heart using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of rat heart using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human kidney using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human kidney using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human liver cancer using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human liver cancer using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of mouse liver using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of mouse liver using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of rat liver using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of rat liver using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human lung cancer using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human lung cancer using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of mouse heart using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of mouse heart using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66127-1-Ig (AK2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human heart using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human heart tissue using AK2 Monoclonal antibody (66127-1-Ig)

    IHC staining of human heart using 66127-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AK2 Monoclonal antibody (66127-1-Ig)

    IF Staining of HeLa using 66127-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AK2 antibody (66127-1-Ig, Clone: 3H2C10 ) at dilution of 1:5024 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AK2 Monoclonal antibody (66127-1-Ig)

    IF Staining of HepG2 using 66127-1-Ig

    Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AK2 Monoclonal antibody (66127-1-Ig)

    IF Staining of HeLa using 66127-1-Ig

    Immunofluorescent analysis of HeLa cells using 66127-1-Ig (AK2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using AK2 Monoclonal antibody (66127-1-Ig)

    FC experiment of HepG2 using 66127-1-Ig

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug AK2 Monoclonal antibody (66127-1-Ig, Clone:3H2C10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human kidney tissue, human liver tissue, human liver cancer tissue, mouse heart tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66127-1-Ig targets AK2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen AK2 fusion protein Ag17911
Full Name adenylate kinase 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC009405
Gene Symbol AK2
Gene ID (NCBI) 204
RRIDAB_2881526
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AK2 antibody 66127-1-IgDownload protocol
IHC protocol for AK2 antibody 66127-1-IgDownload protocol
IF protocol for AK2 antibody 66127-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols