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AIRE Monoclonal antibody

AIRE Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1A3C3

Cat no : 66262-1-Ig

Synonyms

AIRE, AIRE1, APECED, APECED protein, APS1, APSI, autoimmune regulator, PGA1

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using AIRE Monoclonal antibody (66262-1-Ig)

    WB analysis of human spleen using 66262-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig spleen tissue using AIRE Monoclonal antibody (66262-1-Ig)

    WB analysis of pig spleen using 66262-1-Ig

    pig spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using AIRE Monoclonal antibody (66262-1-Ig)

    WB analysis of human spleen using 66262-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66262-1-Ig (AIRE Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIRE Monoclonal antibody (66262-1-Ig)

    IF Staining of HeLa using 66262-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AIRE Monoclonal antibody (66262-1-Ig)

    IF Staining of HeLa using 66262-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using AIRE antibody (66262-1-Ig, Clone: 1A3C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman spleen tissue, pig spleen tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66262-1-Ig targets AIRE in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AIRE fusion protein Ag19103
Full Name autoimmune regulator
Calculated Molecular Weight 545 aa, 58 kDa
Observed Molecular Weight 58-69 kDa
GenBank Accession NumberBC137270
Gene Symbol AIRE
Gene ID (NCBI) 326
RRIDAB_2881649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AIRE antibody 66262-1-IgDownload protocol
IF protocol for AIRE antibody 66262-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols