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AIPL1 Polyclonal antibody

AIPL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15108-1-AP

Synonyms

AIPL1, AIPL2, LCA4

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using AIPL1 Polyclonal antibody (15108-1-AP)

    WB analysis of Y79 using 15108-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SH-SY5Y cells using AIPL1 Polyclonal antibody (15108-1-AP)

    WB analysis of SH-SY5Y using 15108-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using AIPL1 Polyclonal antibody (15108-1-AP)

    WB analysis of mouse brain using 15108-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using AIPL1 Polyclonal antibody (15108-1-AP)

    IP experiment of Y79 using 15108-1-AP

    IP result of anti-AIPL1 (IP:15108-1-AP, 4ug; Detection:15108-1-AP 1:500) with Y79 cells lysate 2400 ug.

  • Immunohistochemistry (IHC) staining of human eye tissue using AIPL1 Polyclonal antibody (15108-1-AP)

    IHC staining of human eye using 15108-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human eye tissue using AIPL1 Polyclonal antibody (15108-1-AP)

    IHC staining of human eye using 15108-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inY79 cells, mouse brain tissue, SH-SY5Y cells
Positive IP detected inY79 cells
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15108-1-AP targets AIPL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIPL1 fusion protein Ag4625
Full Name aryl hydrocarbon receptor interacting protein-like 1
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44 kDa
GenBank Accession NumberBC012055
Gene Symbol AIPL1
Gene ID (NCBI) 23746
RRIDAB_2224995
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AIPL1 antibody 15108-1-APDownload protocol
IHC protocol for AIPL1 antibody 15108-1-APDownload protocol
IP protocol for AIPL1 antibody 15108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Sci Rep

ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis.

Authors - Lei-Lei Gao
View Article
mouseIP

Zygote

ZP3 and AIPL1 participate in GVBD of mouse oocytes by affecting the nuclear membrane localization and maturation of farnesylated prelamin A

Authors - Zhen Jin
View Article