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AIP/ARA9 Polyclonal antibody

AIP/ARA9 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 18176-1-AP

Synonyms

ARA9, AIP, XAP 2, XAP2, FKBP37

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HepG2 using 18176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HepG2 using 18176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HeLa using 18176-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HepG2 using 18176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HepG2 using 18176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HeLa using 18176-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of HepG2 using 18176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of NIH/3T3 using 18176-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    WB analysis of NIH/3T3 using 18176-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human liver cancer using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human liver cancer using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human heart using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human heart using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human heart using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human heart using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human heart using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    IHC staining of human heart using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HeLa cells using AIP/ARA9 Polyclonal antibody (18176-1-AP)

    FC experiment of HeLa using 18176-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug AIP/ARA9 Polyclonal antibody (18176-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18176-1-AP targets AIP/ARA9 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIP/ARA9 fusion protein Ag12869
Full Name aryl hydrocarbon receptor interacting protein
Calculated Molecular Weight 330 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC104797
Gene Symbol AIP
Gene ID (NCBI) 9049
RRIDAB_11182727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AIP/ARA9 antibody 18176-1-APDownload protocol
IHC protocol for AIP/ARA9 antibody 18176-1-APDownload protocol
FC protocol for AIP/ARA9 antibody 18176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

J Clin Invest

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Authors - Frank L. van de Veerdonk
View Article
ratWB,IHC

Heliyon

PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats

Authors - Wei Zhang
View Article