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AIG1 Polyclonal antibody

AIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14468-1-AP

Synonyms

AIG 1, AIG1, androgen induced 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis using 14468-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis of PC-3 using 14468-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse testis tissue using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis of mouse testis using 14468-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis of K-562 using 14468-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of mouse heart tissue using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis of mouse heart using 14468-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of rat ovary tissue using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis of rat ovary using 14468-1-AP

    rat ovary tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using AIG1 Polyclonal antibody (14468-1-AP)

    WB analysis of rat heart using 14468-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AIG1 Polyclonal antibody (14468-1-AP)

    IHC staining of human ovary tumor using 14468-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using AIG1 Polyclonal antibody (14468-1-AP)

    IHC staining of human ovary tumor using 14468-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inK-562 cells, mouse heart tissue, mouse testis tissue, PC-3 cells, rat heart tissue, rat ovary tissue, mouse ovary tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14468-1-AP targets AIG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIG1 fusion protein Ag5836
Full Name androgen-induced 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC025278
Gene Symbol AIG1
Gene ID (NCBI) 51390
RRIDAB_2273794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AIG1 antibody 14468-1-APDownload protocol
IHC protocol for AIG1 antibody 14468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols