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  • KD/KO Validated

AIDA Polyclonal antibody

AIDA Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 23724-1-AP

Synonyms

AIDA, C1orf80, RP11 378J18.7



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse skeletal muscle tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 23724-1-AP is AIDA Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AIDA fusion protein Ag20588
Full Name axin interactor, dorsalization associated
Calculated Molecular Weight 306 aa, 35 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC015535
Gene Symbol AIDA
Gene ID (NCBI) 64853
RRIDAB_2879311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AIDA, also named as C1orf80, acts as a ventralizing factor during embryogenesis. It inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. It's highest expression in the heart and skeletal muscle. AIDA has three isoforms with MW 35 kDa, 16 kDa and 26 kDa.

Protocols

Product Specific Protocols
WB protocol for AIDA antibody 23724-1-APDownload protocol
IHC protocol for AIDA antibody 23724-1-APDownload protocol
IP protocol for AIDA antibody 23724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIP

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
  • KO Validated
mouseWB,IF

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

David (Verified Customer) (02-22-2022)

Unfortunately, the antibody-conjugated magnetic beads did not show a strong signal for the Luminex-based assay.

  • Applications: Other
  • Primary Antibody Dilution: 10ug/mL
  • Cell Tissue Type: Human