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  • KD/KO Validated

AHSA1 Polyclonal antibody

AHSA1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14725-1-AP

Synonyms

p38, C14orf3, AHA1



Tested Applications

Positive WB detected inA431 cells, HeLa cells, mouse brain tissue, HEK-293 cells, A549 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14725-1-AP targets AHSA1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHSA1 fusion protein Ag6482
Full Name AHA1, activator of heat shock 90kDa protein ATPase homolog 1 (yeast)
Calculated Molecular Weight 338 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000321
Gene Symbol AHSA1
Gene ID (NCBI) 10598
RRIDAB_2223965
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AHSA1 antibody 14725-1-APDownload protocol
IHC protocol for AHSA1 antibody 14725-1-APDownload protocol
IF protocol for AHSA1 antibody 14725-1-APDownload protocol
IP protocol for AHSA1 antibody 14725-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Immunol

The Prognostic and Immunotherapeutic Significance of AHSA1 in Pan-Cancer, and Its Relationship With the Proliferation and Metastasis of Hepatocellular Carcinoma.

Authors - Wenli Li
  • KO Validated
humanWB

Cancers (Basel)

AHSA1 Promotes Proliferation and EMT by Regulating ERK/CALD1 Axis in Hepatocellular Carcinoma

Authors - Jiakang Zhang
  • KD Validated
human,mouseWB,IHC

J Hepatocell Carcinoma

AHSA1 Regulates Hepatocellular Carcinoma Progression via the TGF-β/Akt-Cyclin D1/CDK6 Pathway

Authors - Yanjun Gao