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AHNAK2 Polyclonal antibody

AHNAK2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27078-1-AP

Synonyms

AHNAK nucleoprotein 2, AHNAK2, C14orf78, KIAA2019, Protein AHNAK2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using AHNAK2 Polyclonal antibody (27078-1-AP)

    WB analysis of HeLa using 27078-1-AP

    HeLa cells were subjected to Tris-acetate gel system followed by western blot with 27078-1-AP (AHNAK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using AHNAK2 Polyclonal antibody (27078-1-AP)

    IHC staining of human heart using 27078-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27078-1-AP (AHNAK2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using AHNAK2 Polyclonal antibody (27078-1-AP)

    IHC staining of human heart using 27078-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27078-1-AP (AHNAK2 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27078-1-AP targets AHNAK2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHNAK2 fusion protein Ag25895
Full Name AHNAK nucleoprotein 2
Calculated Molecular Weight 617 kDa
GenBank Accession NumberBC049216
Gene Symbol AHNAK2
Gene ID (NCBI) 113146
RRIDAB_2880744
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AHNAK2 antibody 27078-1-APDownload protocol
IHC protocol for AHNAK2 antibody 27078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols