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  • Featured Product
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AHCYL1 Polyclonal antibody

AHCYL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 10658-3-AP

Synonyms

S-adenosylhomocysteine hydrolase-like protein 1, IRBIT, IP(3)Rs binding protein released with IP(3), DC-expressed AHCY-like molecule, DCAL

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using AHCYL1 Polyclonal antibody (10658-3-AP)

    WB analysis of HeLa using 10658-3-AP

    WB result of AHCYL1 antibody (10658-3-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AHCYL1 transfected HeLa cells.

  • Western Blot (WB) analysis of Jurkat cells using AHCYL1 Polyclonal antibody (10658-3-AP)

    WB analysis of Jurkat using 10658-3-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using AHCYL1 Polyclonal antibody (10658-3-AP)

    WB analysis of mouse thymus using 10658-3-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AHCYL1 Polyclonal antibody (10658-3-AP)

    WB analysis of HeLa using 10658-3-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using AHCYL1 Polyclonal antibody (10658-3-AP)

    WB analysis of Jurkat using 10658-3-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10658-3-AP (AHCYL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AHCYL1 Polyclonal antibody (10658-3-AP)

    IP experiment of HeLa using 10658-3-AP

    IP result of anti-AHCYL1 (IP:10658-3-AP, 4ug; Detection:10658-3-AP 1:1000) with HeLa cells lysate 2400ug.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AHCYL1 Polyclonal antibody (10658-3-AP)

    IHC staining of human liver cancer using 10658-3-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using AHCYL1 Polyclonal antibody (10658-3-AP)

    IHC staining of human liver cancer using 10658-3-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10658-3-AP (AHCYL1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHCYL1 Polyclonal antibody (10658-3-AP)

    IF Staining of HeLa using 10658-3-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using AHCYL1 antibody (10658-3-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inJurkat cells, HeLa cells, mouse thymus tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10658-3-AP targets AHCYL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AHCYL1 fusion protein Ag1017
Full Name S-adenosylhomocysteine hydrolase-like 1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC007576
Gene Symbol AHCYL1
Gene ID (NCBI) 10768
RRIDAB_2226013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

S-adenosylhomocysteine hydrolase-like protein 1 (AHCYL1), also known as IP3 receptor-binding protein released with IP3 (IRBIT), regulates IP3-induced Ca2+ release into the cytoplasm of cells(PMID:23145124). This protein is also named as DCAL, XPVKONA and belongs to the adenosylhomocysteinase family. AHCYL1 mRNA and protein are most abundant in the glandular epithelium of adenocarcinoma of cancerous, but not normal and AHCYL1 protein is predominantly in the nucleus(PMID:22826361). It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for AHCYL1 antibody 10658-3-APDownload protocol
IHC protocol for AHCYL1 antibody 10658-3-APDownload protocol
IF protocol for AHCYL1 antibody 10658-3-APDownload protocol
IP protocol for AHCYL1 antibody 10658-3-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigWB

J Agric Food Chem

Maternal Betaine Supplementation during Gestation Enhances Expression of mtDNA-Encoded Genes through D-Loop DNA Hypomethylation in the Skeletal Muscle of Newborn Piglets.

Authors - Yimin Jia
View Article
ratWB

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang
View Article
ratWB

Mol Nutr Food Res

Transgenerational Inheritance of Betaine-Induced Epigenetic Alterations in Estrogen-Responsive IGF-2/IGFBP2 Genes in Rat Hippocampus.

Authors - Yang Yang
View Article
ratWB

Mol Nutr Food Res

Maternal betaine supplementation in rats induces intergenerational changes in hepatic IGF-1 expression and DNA methylation.

Authors - Nannan Zhao
View Article
humanWB

Pharmaceutics

Protective Role of IRBIT on Sodium Bicarbonate Cotransporter-n1 for Migratory Cancer Cells.

Authors - Soyoung Hwang
View Article
chickenWB

J Nutr

Corticosterone-Induced Lipogenesis Activation and Lipophagy Inhibition in Chicken Liver Are Alleviated by Maternal Betaine Supplementation.

Authors - Yun Hu
View Article