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AHCY Monoclonal antibody

AHCY Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F9B10

Cat no : 66019-1-Ig

Synonyms

Adenosylhomocysteinase, AdoHcyase, AHCY, S adenosylhomocysteine hydrolase, SAHH

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis using 66019-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of human testis using 66019-1-Ig

    human testis tissue were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of HeLa using 66019-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of MCF-7 using 66019-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of L02 cells using AHCY Monoclonal antibody (66019-1-Ig)

    WB analysis of L02 using 66019-1-Ig

    L02 cells were subjected to SDS PAGE followed by western blot with 66019-1-Ig (AHCY antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human colon cancer using 66019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human colon cancer using 66019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66019-1-Ig (AHCY antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human testis tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human testis using 66019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human testis using 66019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human testis using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human placenta using 66019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using AHCY Monoclonal antibody (66019-1-Ig)

    IHC staining of human placenta using 66019-1-Ig

    Immunohistochemical analysis of paraffin-embedded human placenta using 66019-1-Ig(AHCY antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AHCY Monoclonal antibody (66019-1-Ig)

    IF Staining of HeLa using 66019-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using AHCY antibody (66019-1-Ig, Clone: 1F9B10 ) at dilution of 1:4000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, human testis tissue, L02 cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66019-1-Ig targets AHCY in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen AHCY fusion protein Ag18036
Full Name S-adenosylhomocysteine hydrolase
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC011606
Gene Symbol AHCY
Gene ID (NCBI) 191
RRIDAB_11044194
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AHCY, also named as SAHH, belongs to the adenosylhomocysteinase family. AHCY is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore AHCY may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. AHCY catalyzes the reversible hydrolysis of S-adenosylhomocysteine to adenosine and L-homocysteine.(PMID:21220475).

Protocols

Product Specific Protocols
WB protocol for AHCY antibody 66019-1-IgDownload protocol
IHC protocol for AHCY antibody 66019-1-IgDownload protocol
IF protocol for AHCY antibody 66019-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation

Authors - Yankun Wang
View Article