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AGXT2L2 Polyclonal antibody

AGXT2L2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16449-1-AP

Synonyms

5-phosphohydroxy-L-lysine phospho-lyase, AGXT2L2, PHYKPL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGXT2L2 Polyclonal antibody (16449-1-AP)

    WB analysis using 16449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16449-1-AP (AGXT2L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AGXT2L2 Polyclonal antibody (16449-1-AP)

    IHC staining of human prostate cancer using 16449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16449-1-AP (AGXT2L2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using AGXT2L2 Polyclonal antibody (16449-1-AP)

    IHC staining of human prostate cancer using 16449-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16449-1-AP (AGXT2L2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse heart tissue, rat heart
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16449-1-AP targets AGXT2L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGXT2L2 fusion protein Ag8391
Full Name alanine-glyoxylate aminotransferase 2-like 2
Calculated Molecular Weight450aa,50 kDa; 175aa,19 kDa
GenBank Accession NumberBC008009
Gene Symbol AGXT2L2
Gene ID (NCBI) 85007
RRIDAB_2225868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGXT2L2 antibody 16449-1-APDownload protocol
IHC protocol for AGXT2L2 antibody 16449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols