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AGXT Polyclonal antibody

AGXT Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22394-1-AP

Synonyms

AGT, AGT1, AGXT, AGXT1, PH1, SPAT, SPT, TLH6

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using AGXT Polyclonal antibody (22394-1-AP)

    WB analysis of mouse liver using 22394-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using AGXT Polyclonal antibody (22394-1-AP)

    WB analysis of mouse lung using 22394-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 22394-1-AP (AGXT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inmouse liver tissue, mouse lung tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

22394-1-AP targets AGXT in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGXT fusion protein Ag18062
Full Name alanine-glyoxylate aminotransferase
Calculated Molecular Weight 392 aa, 43 kDa
Observed Molecular Weight 43-45 kDa
GenBank Accession NumberBC132819
Gene Symbol AGXT
Gene ID (NCBI) 189
RRIDAB_2879098
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGXT (AGT) is a key enzyme in glyoxylate detoxification. Mutations of AGXT lead to primary hyperoxaluria type I, a rare metabolic disease which is characterized by deficient AGXT, excessive hepatic oxalate production that leads to renal failure.

Protocols

Product Specific Protocols
WB protocol for AGXT antibody 22394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nucleic Acid Ther

Systemic Alanine Glyoxylate Aminotransferase Messenger RNA Improves Glyoxylate Metabolism in a Mouse Model of Primary Hyperoxaluria Type 1.

Authors - Anjli Kukreja
View Article