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  • KD/KO Validated

AGTR1 Polyclonal antibody

AGTR1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 25343-1-AP

Synonyms

AT1, Angiotensin II type-1 receptor, Angiotensin II type 1 receptor, AGTR1B, AGTR1A



Tested Applications

Positive WB detected inC2C12 cells, PC-12 cells, mouse kidney tissue, mouse heart tissue, HuH-7 cells, HEK-293 cells
Positive IHC detected inmouse heart tissue, human liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25343-1-AP targets AGTR1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGTR1 fusion protein Ag14461
Full Name angiotensin II receptor, type 1
Calculated Molecular Weight 359 aa, 41 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC022447
Gene Symbol AGTR1
Gene ID (NCBI) 185
RRIDAB_2880037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Angiotensin II (Ang II), the main effector molecule of the renin-angiotensin system, exerts its actions mainly via interaction with type-1 angiotensin II receptor (AGTR1, also named AT1R), thereby contributing to blood pressure regulation. AGTR1 mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. By regulating vascular tone, cardiovascular function, and salt and water homeostasis, AGTR1 exerts an indispensable physiological role (PMID: 21600887). AGTR1 has been implicated in diverse aspects of human disease, from the regulation of blood pressure and cardiovascular homeostasis to cancer progression (PMID: 26975580).

Protocols

Product Specific Protocols
WB protocol for AGTR1 antibody 25343-1-APDownload protocol
IHC protocol for AGTR1 antibody 25343-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,CoIP

Cell Res

Cartilage oligomeric matrix protein is an endogenous β-arrestin-2-selective allosteric modulator of AT1 receptor counteracting vascular injury.

Authors - Yi Fu
mouseWB

Aging Cell

Activation of angiotensin-converting enzyme 2/angiotensin (1-7)/mas receptor axis triggers autophagy and suppresses microglia proinflammatory polarization via forkhead box class O1 signaling

Authors - Ruili Dang
humanIF

Biomaterials

Inhibition of tumor-promoting stroma to enforce subsequently targeting AT1R on tumor cells by pathological inspired micelles.

Authors - Yun Zhu
mouseWB

Redox Biol

Vitamin D receptor activation regulates microglia polarization and oxidative stress in spontaneously hypertensive rats and angiotensin II-exposed microglial cells: Role of renin-angiotensin system.

Authors - Changmeng Cui
zebrafishWB,IF

Elife

A zebrafish screen reveals Renin-angiotensin system inhibitors as neuroprotective via mitochondrial restoration in dopamine neurons.

Authors - Gha-Hyun J Kim
  • KD Validated
mouseWB

Int J Mol Sci

Exercise Normalized the Hippocampal Renin-Angiotensin System and Restored Spatial Memory Function, Neurogenesis, and Blood-Brain Barrier Permeability in the 2K1C-Hypertensive Mouse.

Authors - Ying-Shuang Chang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kazu (Verified Customer) (12-14-2022)

This antibody worked well on mouse optic nerve sections at optimized dilution of 1:100.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Optic nerve
FH

Wu (Verified Customer) (07-16-2021)

Works good for IHC assay

  • Applications: Immunohistochemistry
  • Cell Tissue Type: glandular lumen
AGTR1 Antibody Immunohistochemistry validation ( dilution) in glandular lumen (Cat no:25343-1-AP)