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AGR2 Polyclonal antibody

AGR2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12275-1-AP

Synonyms

GOB4, GOB 4, AGR 2, AG-2, AG2



Tested Applications

Positive WB detected inA549 cells, rat colon tissue, rat stomach tissue, mouse stomach tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12275-1-AP targets AGR2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGR2 fusion protein Ag2919
Full Name anterior gradient homolog 2 (Xenopus laevis)
Calculated Molecular Weight 175 aa, 20 kDa
Observed Molecular Weight 17-20 kDa
GenBank Accession NumberBC015503
Gene Symbol AGR2
Gene ID (NCBI) 10551
RRIDAB_2225096
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGR2, also named AG2 or HPC8, encodes anterior gradient protein 2 homolog which belongs to the AGR family. It is a secreted protein localized in endoplasmic reticulum. AGR2 plays roles in MUC2 post-transcriptional synthesis,secretion and production of mucus by intestinal cells. AGR2 was significantly elevated in the pancreatic juice from patients with pre-malignant conditions as well as pancreatic cancer compared to control pancreatic juice samples. AGR2 levels in pancreatic juice could potentially be used in assessment of high-risk patients undergoing endoscopic procedures.

Protocols

Product Specific Protocols
WB protocol for AGR2 antibody 12275-1-APDownload protocol
IHC protocol for AGR2 antibody 12275-1-APDownload protocol
IF protocol for AGR2 antibody 12275-1-APDownload protocol
IP protocol for AGR2 antibody 12275-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia.

Authors - Cindrilla Chumduri
human,mouseIHC

Nat Protoc

Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.

Authors - Rajendra Kumar Gurumurthy
humanIHC

Cell Rep Med

Distinctive multicellular immunosuppressive hubs confer different intervention strategies for left- and right-sided colon cancers

Authors - Bingxin Liu
humanWB,IHC

Proc Natl Acad Sci U S A

A discrete population of squamocolumnar junction cells implicated in the pathogenesis of cervical cancer.

Authors - Herfs Michael M
humanIHC

J Pathol

A novel blueprint for 'top down' differentiation defines the cervical squamocolumnar junction during development, reproductive life, and neoplasia.

Authors - Michael Herfs
humanIHC

Int J Cancer

Unique recurrence patterns of cervical intraepithelial neoplasia following excision of the squamo-columnar junction.

Authors - Michael Herfs