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  • KD/KO Validated

GPAT3 Polyclonal antibody

GPAT3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 20603-1-AP

Synonyms

AGPAT9, 1-AGPAT 9, 1-AGP acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 9, 1-acyl-sn-glycerol-3-phosphate O-acyltransferase 10



Tested Applications

Positive WB detected inrat kidney tissue, HeLa cells, HT-1080 cells
Positive IHC detected inhuman kidney tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20603-1-AP targets GPAT3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GPAT3 fusion protein Ag14579
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 9
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC006236
Gene Symbol AGPAT9
Gene ID (NCBI) 84803
RRIDAB_10694289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GPAT3, also known as AGPAT9, is a member of the lysophosphatidic acid acyltransferase protein family. GPAT3 is an enzyme that catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol.

Protocols

Product Specific Protocols
WB protocol for GPAT3 antibody 20603-1-APDownload protocol
IHC protocol for GPAT3 antibody 20603-1-APDownload protocol
IF protocol for GPAT3 antibody 20603-1-APDownload protocol
FC protocol for GPAT3 antibody 20603-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi
mouseWB

Cell Metab

AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.

Authors - Hui Luo
mouseWB

Hum Mol Genet

PMID: 31873720

Authors - Mingming Gao
  • KO Validated
mouseWB,IF

Cell Death Dis

GPAT3 regulates the synthesis of lipid intermediate LPA and exacerbates Kupffer cell inflammation mediated by the ERK signaling pathway

Authors - Guoqiang Fan
humanWB

Ecotoxicol Environ Saf

Perturbation of arachidonic acid and glycerolipid metabolism promoted particulate matter-induced inflammatory responses in human bronchial epithelial cells

Authors - Jian Wang
  • KD Validated
mouseWB

Biomed Pharmacother

Si-Ni-San reverses dietary fat absorption defects in a murine model of depression

Authors - Yuanyuan Liu