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AGPAT6 Polyclonal antibody

AGPAT6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16762-1-AP

Synonyms

1 AGP acyltransferase 6, 1 AGPAT 6, AGPAT6, GPAT4, LPAAT zeta, LPAATZ, TSARG7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AGPAT6 Polyclonal antibody (16762-1-AP)

    WB analysis using 16762-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    WB analysis of mouse testis using 16762-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16762-1-AP (AGPAT6 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IP experiment of mouse brain using 16762-1-AP

    IP result of anti-AGPAT6 (IP:16762-1-AP, 4ug; Detection:16762-1-AP 1:1000) with mouse brain tissue lysate 2640ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human testis using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human testis using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human skin using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human skin using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human liver using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human liver using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human ovary using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human ovary using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human kidney using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AGPAT6 Polyclonal antibody (16762-1-AP)

    IHC staining of human kidney using 16762-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16762-1-AP (AGPAT6 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse brain tissue, mouse testis tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman testis tissue, human kidney tissue, human liver tissue, human ovary tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

16762-1-AP targets AGPAT6 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGPAT6 fusion protein Ag10294
Full Name 1-acylglycerol-3-phosphate O-acyltransferase 6 (lysophosphatidic acid acyltransferase, zeta)
Calculated Molecular Weight 456 aa, 52 kDa
Observed Molecular Weight52 kDa
GenBank Accession NumberBC051377
Gene Symbol AGPAT6
Gene ID (NCBI) 137964
RRIDAB_1850878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AGPAT6 is a member of the 1-acylglycerol-3-phosphate O-acyltransferase (AGPAT) family. AGPAT6 is expressed in brown adipose tissue and mammary epithelium as well as many other tissues. A deficiency of AGPAT6 in mice results in resistance to dietinduced and genetic forms of obesity.

Protocols

Product Specific Protocols
WB protocol for AGPAT6 antibody 16762-1-APDownload protocol
IHC protocol for AGPAT6 antibody 16762-1-APDownload protocol
IP protocol for AGPAT6 antibody 16762-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Invest Ophthalmol Vis Sci

Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.

Authors - Sasha M Milbeck
View Article
mouseWB

EMBO Rep

MTCH2 cooperates with MFN2 and lysophosphatidic acid synthesis to sustain mitochondrial fusion

Authors - Andres Goldman
View Article