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AGFG2 Polyclonal antibody

AGFG2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11919-1-AP

Synonyms

RABR, RAB R, HRBL, HIV-1 Rev-binding protein-like protein, ArfGAP with FG repeats 2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using AGFG2 Polyclonal antibody (11919-1-AP)

    WB analysis of Jurkat using 11919-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using AGFG2 Polyclonal antibody (11919-1-AP)

    WB analysis of Jurkat using 11919-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using AGFG2 Polyclonal antibody (11919-1-AP)

    WB analysis of K-562 using 11919-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11919-1-AP (AGFG2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using AGFG2 Polyclonal antibody (11919-1-AP)

    IHC staining of human lymphoma using 11919-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma using 11919-1-AP (AGFG2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AGFG2 Polyclonal antibody (11919-1-AP)

    IF Staining of HepG2 using 11919-1-AP

    Immunofluorescent analysis of HepG2 cells, using AGFG2 antibody 11919-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inJurkat cells, K-562 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11919-1-AP targets AGFG2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGFG2 fusion protein Ag2546
Full Name ArfGAP with FG repeats 2
Calculated Molecular Weight 481 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC017329
Gene Symbol AGFG2
Gene ID (NCBI) 3268
RRIDAB_2224646
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGFG2 antibody 11919-1-APDownload protocol
IHC protocol for AGFG2 antibody 11919-1-APDownload protocol
IF protocol for AGFG2 antibody 11919-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols