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AGAP2 Polyclonal antibody

AGAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14690-1-AP

Synonyms

Cnt-g1, CENTG1, Centaurin-gamma-1, Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2, AGAP-2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human brain tissue using AGAP2 Polyclonal antibody (14690-1-AP)

    WB analysis of human brain using 14690-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using AGAP2 Polyclonal antibody (14690-1-AP)

    WB analysis of rat brain using 14690-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using AGAP2 Polyclonal antibody (14690-1-AP)

    WB analysis of rat brain using 14690-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 14690-1-AP (AGAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using AGAP2 Polyclonal antibody (14690-1-AP)

    IP experiment of mouse brain using 14690-1-AP

    IP result of anti-AGAP2 (IP:14690-1-AP, 4ug; Detection:14690-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using AGAP2 Polyclonal antibody (14690-1-AP)

    IHC staining of human gliomas using 14690-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using AGAP2 Polyclonal antibody (14690-1-AP)

    IHC staining of human gliomas using 14690-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14690-1-AP (AGAP2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AGAP2 Polyclonal antibody (14690-1-AP)

    IF Staining of HeLa using 14690-1-AP

    Immunofluorescent analysis of Hela cells, using AGAP2 antibody 14690-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inhuman brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14690-1-AP targets AGAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AGAP2 fusion protein Ag6142
Full Name ArfGAP with GTPase domain, ankyrin repeat and PH domain 2
Calculated Molecular Weight 125 kDa
Observed Molecular Weight 124 kDa
GenBank Accession NumberBC028020
Gene Symbol AGAP2
Gene ID (NCBI) 116986
RRIDAB_2224214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for AGAP2 antibody 14690-1-APDownload protocol
IHC protocol for AGAP2 antibody 14690-1-APDownload protocol
IF protocol for AGAP2 antibody 14690-1-APDownload protocol
IP protocol for AGAP2 antibody 14690-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Phytomedicine

Demethylzeylasteral attenuates hepatic stellate cell activation and liver fibrosis by inhibiting AGAP2 mediated signaling.

Authors - Ke Chen
View Article