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  • Featured Product
  • KD/KO Validated

AFG3L2 Polyclonal antibody

AFG3L2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14631-1-AP

Synonyms

Paraplegin-like protein, Mitochondrial inner membrane m-AAA protease component AFG3L2, EC:3.4.24.-, AFG3-like protein 2, AFG3L 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis using 14631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis using 14631-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of HeLa using 14631-1-AP

    WB result of AFG3L2 antibody (14631-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AFG3L2 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of mouse skeletal muscle using 14631-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    WB analysis of mouse kidney using 14631-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14631-1-AP (AFG3L2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IP experiment of HeLa using 14631-1-AP

    IP result of anti-AFG3L2 (IP:14631-1-AP, 4ug; Detection:14631-1-AP 1:5000) with HeLa cells lysate 1560 ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14631-1-AP (AFG3L2 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using AFG3L2 Polyclonal antibody (14631-1-AP)

    IHC staining of human kidney using 14631-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IF Staining of HepG2 using 14631-1-AP

    Immunofluorescent analysis of HepG2 cells, using AFG3L2 antibody 14631-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using AFG3L2 Polyclonal antibody (14631-1-AP)

    IF Staining of HeLa using 14631-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14631-1-AP (AFG3L2 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse skeletal muscle tissue, mouse kidney tissue, Jurkat cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14631-1-AP targets AFG3L2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AFG3L2 fusion protein Ag6209
Full Name AFG3 ATPase family gene 3-like 2 (yeast)
Calculated Molecular Weight 88 kDa
Observed Molecular Weight 80-90 kDa
GenBank Accession NumberBC065016
Gene Symbol AFG3L2
Gene ID (NCBI) 10939
RRIDAB_2242420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly(PMID:17101804). Human AFG3L2 is an 80-kDa protein encoded by a 17-exon gene and highly and selectively expressed in human cerebellar Purkinje cells(PMID:20208537) and it can exsit as a truncated 65 kDa protein(PMID:18337413). Defects in AFG3L2 are the cause of spinocerebellar ataxia type 28 (SCA28) and spastic ataxia autosomal recessive type 5 (SPAX5).

Protocols

Product Specific Protocols
WB protocol for AFG3L2 antibody 14631-1-APDownload protocol
IHC protocol for AFG3L2 antibody 14631-1-APDownload protocol
IF protocol for AFG3L2 antibody 14631-1-APDownload protocol
IP protocol for AFG3L2 antibody 14631-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Cell

Mechanisms and significance of tissue-specific MICU regulation of the mitochondrial calcium uniporter complex

Authors - Chen-Wei Tsai
  • KD Validated
View Article
mouseWB

Sci Adv

Adult-onset obesity is triggered by impaired mitochondrial gene expression.

Authors - Kara L Perks
View Article
WB

Sci Adv

Fidelity of translation initiation is required for coordinated respiratory complex assembly.

Authors - Danielle L Rudler
View Article
humanIF

Cancer Res

Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition

Authors - Liang Fang
View Article
mouseWB

Research (Wash D C)

LonP1 Links Mitochondria-ER Interaction to Regulate Heart Function

Authors - Yujie Li
View Article
humanWB

Brain

Mutations in the SPG7 gene cause chronic progressive external ophthalmoplegia through disordered mitochondrial DNA maintenance.

Authors - Gerald Pfeffer
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Boyan (Verified Customer) (04-28-2023)

clean band around the expected size

  • Applications: Western Blot