AFAP1L1 Polyclonal antibody
AFAP1L1 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, monkey
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 23909-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | COS-7 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 23909-1-AP is AFAP1L1 Fusion Protein expressed in E. coli.
Tested Reactivity | human, monkey |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | AFAP1L1 fusion protein Ag21006 |
Full Name | actin filament associated protein 1-like 1 |
Calculated Molecular Weight | 768 aa, 86 kDa |
Observed Molecular Weight | 75-86 kDa |
GenBank Accession Number | BC125093 |
Gene Symbol | AFAP1L1 |
Gene ID (NCBI) | 134265 |
RRID | AB_2879358 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen Affinity purified |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Actin Filament-Associated Protein of 110 kDa (AFAP-110/AFAP1) is an adaptor protein with multiple protein binding motifs that is known to function as both an actin binding protein and a cSrc activating protein. A homology search of AFAP1 recently identified AFAP1L1 which has a similar sequence, domain structure and cellular localization. AFAP1L1 is highly expressed in human breast, colon and brain tissue. AFAP1L1 interacts with cortactin and involves the invadosomes formation. AFAP1L1 as a prognostic marker has a role in the progression of spindle cell sarcomas.
Protocols
Product Specific Protocols | |
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WB protocol for AFAP1L1 antibody 23909-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |