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AEBP2 Monoclonal antibody

AEBP2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

7H2B11

Cat no : 66141-1-Ig

Synonyms

AE binding protein 2, AEBP2, Zinc finger protein AEBP2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using AEBP2 Monoclonal antibody (66141-1-Ig)

    WB analysis of HeLa using 66141-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66141-1-Ig (AEBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 66141-1-Ig is AEBP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen AEBP2 fusion protein Ag17905
Full Name AE binding protein 2
Calculated Molecular Weight 33 kDa, 54 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC015624
Gene Symbol AEBP2
Gene ID (NCBI) 121536
RRIDAB_2881538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AEBP2 belongs to the AEBP2/jing C2H2-type zinc-finger family. It is a DNA-binding transcriptional repressor. AEBP2 is a zinc finger protein that has been shown to interact with the mammalian Polycomb Repression Complex 2 (PRC2). It may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. AEBP2 is an evolutionarily well-conserved gene that is found in the animals ranging from flying insects to mammals. (PMID:19293275) The antibody recognizes adult-specific larger form (52 kDa) and the embryo-specific smaller form (31 kDa).

Protocols

Product Specific Protocols
WB protocol for AEBP2 antibody 66141-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols