AEBP2 Polyclonal antibody

AEBP2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 11232-2-AP

Synonyms

AE binding protein 2, AEBP2, Zinc finger protein AEBP2



Tested Applications

Positive WB detected inhuman testis tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11232-2-AP targets AEBP2 in WB, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen AEBP2 fusion protein Ag1756
Full Name AE binding protein 2
Calculated Molecular Weight 33 kDa, 54 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015624
Gene Symbol AEBP2
Gene ID (NCBI) 121536
RRIDAB_2225962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AEBP2, also named as Zinc finger protein AEBP2 or Adipocyte enhancer-binding protein 2, is a 517 amino acid protein, which contains 3 C2H2-type zinc fingers and belongs to the AEBP2/jing C2H2-type zinc-finger family. AEBP2 as a DNA-binding transcriptional repressor may interact with and stimulate the activity of the PRC2 complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. It may e playing a role in craniofacial and digital development, as well as development of the central nervous system and gastrointestinal tract.

Protocols

Product Specific Protocols
WB protocol for AEBP2 antibody 11232-2-APDownload protocol
IP protocol for AEBP2 antibody 11232-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

Dual functions of Tet1 in transcriptional regulation in mouse embryonic stem cells.

Authors - Wu Hao H
humanWB

Mol Cancer

Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer

Authors - Rashmi Bharti
human

PLoS Genet

Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks.

Authors - Holdt Lesca M LM
mouseWB,ChIP

Nucleic Acids Res

AEBP2 as a potential targeting protein for Polycomb Repression Complex PRC2.

Authors - Kim Hana H
mouseWB

PLoS One

Aebp2 as an epigenetic regulator for neural crest cells.

Authors - Kim Hana H