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ADSS Polyclonal antibody

ADSS Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 16373-1-AP

Synonyms

ADEH, adenylosuccinate synthase, ADSS, AdSS 2, ADSS2, AMPSase 2, IMP aspartate ligase 2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ADSS Polyclonal antibody (16373-1-AP)

    WB analysis of mouse skeletal muscle using 16373-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16373-1-AP (ADSS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using ADSS Polyclonal antibody (16373-1-AP)

    WB analysis of mouse skeletal muscle using 16373-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16373-1-AP (ADSS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using ADSS Polyclonal antibody (16373-1-AP)

    IP experiment of mouse testis using 16373-1-AP

    IP result of anti-ADSS (IP:16373-1-AP, 4ug; Detection:16373-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

Tested Applications

Positive WB detected inmouse skeletal muscle tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16373-1-AP targets ADSS in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADSS fusion protein Ag9298
Full Name adenylosuccinate synthase
Calculated Molecular Weight 456 aa, 50 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC012356
Gene Symbol ADSS
Gene ID (NCBI) 159
RRIDAB_2225676
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADSS antibody 16373-1-APDownload protocol
IP protocol for ADSS antibody 16373-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.

Authors - Eunus S Ali
View Article
humanWB

Cell Rep

The mTORC1 Signaling Network Senses Changes in Cellular Purine Nucleotide Levels.

Authors - Gerta Hoxhaj
View Article
humanWB, IHC

PLoS Biol

Nucleotide de novo synthesis increases breast cancer stemness and metastasis via cGMP-PKG-MAPK signaling pathway.

Authors - Yajing Lv
View Article
human,mouseWB

Cell

De novo and salvage purine synthesis pathways across tissues and tumors

Authors - Diem H Tran
View Article