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- KD/KO Validated
ADRM1 Polyclonal antibody
ADRM1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 11468-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, K-562 cells, PC-3 cells, Raji cells, NIH/3T3 cells |
Positive IP detected in | mouse testis tissue |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 3 publications below |
WB | See 6 publications below |
IHC | See 5 publications below |
IF | See 3 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
11468-1-AP targets ADRM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ADRM1 fusion protein Ag1997 |
Full Name | adhesion regulating molecule 1 |
Calculated Molecular Weight | 407 aa, 42 kDa |
Observed Molecular Weight | 46 kDa |
GenBank Accession Number | BC017245 |
Gene Symbol | ADRM1 |
Gene ID (NCBI) | 11047 |
RRID | AB_2273873 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The 26S proteasome is a key component of the ubiquitin-proteasome system, a process responsible for the majority of cellular protein degradation. hRpn13 (also termed ADRM1 or GP110) is a novel 46-kDa subunit of its 19S regulatory complex. hRpn13 binds directly to the proteasome-associated deubiquitinating enzyme, UCH37, and enhances its isopeptidase activity. Overexpression of hRpn13 promotes the activity of the ubiquitin-proteasome system and modulates the influence of osteoblasts on osteoclasts by controlling the stability of regulatory proteins in osteoblasts.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for ADRM1 antibody 11468-1-AP | Download protocol |
IHC protocol for ADRM1 antibody 11468-1-AP | Download protocol |
IF protocol for ADRM1 antibody 11468-1-AP | Download protocol |
IP protocol for ADRM1 antibody 11468-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A Dendritic spinopathy in transgenic mice expressing ALS/dementia-linked mutant UBQLN2. | ||
Oncogene The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a. | ||
Genes Chromosomes Cancer Comprehensive analysis of 20q13 genes in ovarian cancer identifies ADRM1 as amplification target. | ||
Biochem Biophys Res Commun Disturbance of proteasomal and autophagic protein degradation pathways by amyotrophic lateral sclerosis-linked mutations in ubiquilin 2. |