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ADRA2B-Specific Polyclonal antibody

ADRA2B-Specific Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19778-1-AP

Synonyms

ADRA2B, Alpha 2B adrenoceptor, Alpha 2B adrenergic receptor, ADRARL1, ADRA2RL1

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human gliomas tissue using ADRA2B-Specific Polyclonal antibody (19778-1-AP)

    IHC staining of human gliomas using 19778-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19778-1-AP (ADRA2B-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ADRA2B-Specific Polyclonal antibody (19778-1-AP)

    IHC staining of human gliomas using 19778-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19778-1-AP (ADRA2B-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19778-1-AP targets ADRA2B-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name adrenergic, alpha-2B-, receptor
Calculated Molecular Weight 50 kDa
GenBank Accession NumberNM_000682
Gene Symbol ADRA2B
Gene ID (NCBI) 151
RRIDAB_10640910
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRA2B, also named as ADRA2L1 and ADRA2RL1, belongs to the G-protein coupled receptor 1 family. ADRA2B mediates the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins. The antibody is specific to ADRA2B.

Protocols

Product Specific Protocols
IHC protocol for ADRA2B-Specific antibody 19778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Cell Tissue Res

Subcellular distribution of α2-adrenoceptor subtypes in the rodent kidney.

Authors - Takaomi Shimokawa
View Article
ratWB

Eur J Pharmacol

Yohimbine ameliorates lipopolysaccharide-induced acute kidney injury in rats.

Authors - Takaomi Shimokawa
View Article
ratWB,IF

J Pharmacol Sci

Inhibition of α2-adrenoceptor is renoprotective in 5/6 nephrectomy-induced chronic kidney injury rats.

Authors - Kohei Hayashi
View Article