ADRA2A Polyclonal antibody

ADRA2A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14266-1-AP

Synonyms

Alpha 2A adrenoreceptor, Alpha 2A adrenoceptor, Alpha 2A adrenergic receptor, ADRAR, ADRA2R



Tested Applications

Positive WB detected inPC-12 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14266-1-AP targets ADRA2A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADRA2A fusion protein Ag5562
Full Name adrenergic, alpha-2A-, receptor
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC050414
Gene Symbol ADRA2A
Gene ID (NCBI) 150
RRIDAB_2636822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADRA2A (Adrenoceptor Alpha-2) belongs to the adrenoceptor alpha-2 family of GPCRs coupled to the inhibitory G-alpha subunit (Gi) that suppresses adenylyl cyclase activity, thereby suppressing the cyclization of AMP to cAMP (PMID: 38132444). ADRA2A inhibits the flow of sympathetic nerves in the central nervous system and plays a major role in sedation, anxiolysis, and antinociception (PMID: 33915198).

Protocols

Product Specific Protocols
WB protocol for ADRA2A antibody 14266-1-APDownload protocol
IHC protocol for ADRA2A antibody 14266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biomed Pharmacother

Dexmedetomidine facilitates autophagic flux to promote liver regeneration by suppressing GSK3β activity in mouse partial hepatectomy

Authors - Xueya Yao
ratWB

Int J Mol Sci

Chronic Administrations of Guanfacine on Mesocortical Catecholaminergic and Thalamocortical Glutamatergic Transmissions.

Authors - Kouji Fukuyama
humanWB

Am J Cancer Res

SNHG15, a p53-regulated lncRNA, suppresses cisplatin-induced apoptosis and ROS accumulation through the miR-335-3p/ZNF32 axis.

Authors - Yue-Feng Sun
mouseIHC

Front Immunol

β2-Adrenoceptor Deficiency Results in Increased Calcified Cartilage Thickness and Subchondral Bone Remodeling in Murine Experimental Osteoarthritis.

Authors - Gundula Rösch
mouseWB

Front Mol Neurosci

PMID: 28955200

Authors - Yong-Jie Wang
mouseWB

Exp Neurol

Two-hour acute restraint stress facilitates escape behavior and learning outcomes through the activation of the Cdk5/GR P S211 pathway in male mice.

Authors - Bin Wang