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ADPGK Polyclonal antibody

ADPGK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 15639-1-AP

Synonyms

2610017G09Rik, ADP dependent glucokinase, ADP GK, ADPGK, RbBP 35

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADPGK Polyclonal antibody (15639-1-AP)

    WB analysis using 15639-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15639-1-AP (ADPGK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ADPGK Polyclonal antibody (15639-1-AP)

    IHC staining of human liver using 15639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADPGK Polyclonal antibody (15639-1-AP)

    IHC staining of human liver using 15639-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 15639-1-AP (ADPGK antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADPGK Polyclonal antibody (15639-1-AP)

    IF Staining of HepG2 using 15639-1-AP

    Immunofluorescent analysis of HepG2 cells using 15639-1-AP (ADPGK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, MOLT-4 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15639-1-AP targets ADPGK in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADPGK fusion protein Ag8105
Full Name ADP-dependent glucokinase
Calculated Molecular Weight 497 aa, 54 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC006112
Gene Symbol ADPGK
Gene ID (NCBI) 83440
RRIDAB_2224911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADP-dependent glucokinase (ADPGK) has frst been described 1994 in hyperthermophilic archaea as a novel glucose-phosphorylating enzyme dependent on ADP (adenosine diphosphate) instead of ATP (adenosine triphosphate). Highest ADPGK expression is found in immune cells of both myeloid and lymphoid lineages. Catalyzes the phosphorylation of D-glucose to D-glucose 6-phosphate using ADP as the phosphate donor. GDP and CDP can replace ADP, but with reduced efficiency (By similarity).

Protocols

Product Specific Protocols
WB protocol for ADPGK antibody 15639-1-APDownload protocol
IHC protocol for ADPGK antibody 15639-1-APDownload protocol
IF protocol for ADPGK antibody 15639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF

Front Mol Neurosci

Quantitative Proteomic Analysis of Mouse Sciatic Nerve Reveals Post-injury Upregulation of ADP-Dependent Glucokinase Promoting Macrophage Phagocytosis.

Authors - Kai Zhang
View Article
humanWB

Res Sq

Molecular and clinical characterization of a founder mutation causing G6PC3 deficiency

Authors - Xin Zhen
View Article