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ADNP Polyclonal antibody

ADNP Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 17987-1-AP

Synonyms

KIAA0784, ADNP1, Activity-dependent neuroprotector homeobox protein, Activity-dependent neuroprotective protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ADNP Polyclonal antibody (17987-1-AP)

    WB analysis using 17987-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17987-1-AP (ADNP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADNP Polyclonal antibody (17987-1-AP)

    WB analysis using 17987-1-AP

    HEK-293T cells were subjected to SDS PAGE followed by western blot with 17987-1-AP (ADNP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ADNP Polyclonal antibody (17987-1-AP)

    IF Staining of HeLa using 17987-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ADNP antibody (17987-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17987-1-AP targets ADNP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADNP fusion protein Ag12414
Full Name activity-dependent neuroprotector homeobox
Calculated Molecular Weight 1102 aa, 123 kDa
Observed Molecular Weight 135-145 kDa
GenBank Accession NumberBC075794
Gene Symbol ADNP
Gene ID (NCBI) 23394
RRIDAB_2222383
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADNP antibody 17987-1-APDownload protocol
IF protocol for ADNP antibody 17987-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Front Oncol

ADNP Upregulation Promotes Bladder Cancer Cell Proliferation via the AKT Pathway.

Authors - Shuai Zhu
View Article
mouseIHC

J Cancer

ADNP prompts the cisplatin-resistance of bladder cancer via TGF-β-mediated epithelial-mesenchymal transition (EMT) pathway.

Authors - Yu Xie
View Article
humanIF

Mol Autism

Clinical impact and in vitro characterization of ADNP variants in pediatric patients

Authors - Chuanhui Ge
View Article