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ADK Polyclonal antibody

ADK Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15477-1-AP

Synonyms

Adenosine 5 phosphotransferase, adenosine kinase, ADK, AK

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using ADK Polyclonal antibody (15477-1-AP)

    WB analysis using 15477-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15477-1-AP (ADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Polyclonal antibody (15477-1-AP)

    IHC staining of human liver cancer using 15477-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ADK Polyclonal antibody (15477-1-AP)

    IHC staining of human liver cancer using 15477-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15477-1-AP (ADK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, PC-3 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15477-1-AP targets ADK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADK fusion protein Ag7783
Full Name adenosine kinase
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 40-46 kDa
GenBank Accession NumberBC003568
Gene Symbol ADK
Gene ID (NCBI) 132
RRIDAB_2222085
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADK antibody 15477-1-APDownload protocol
IHC protocol for ADK antibody 15477-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Neurochem Res

NLRP3 Inflammasome Activation Enhances ADK Expression to Accelerate Epilepsy in Mice

Authors - Haiju Zhang
View Article