Adiponectin Polyclonal antibody

Name: Adiponectin Polyclonal antibody
Brand: Proteintech
SKU: 21613-1-AP

Adiponectin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 21613-1-AP

Synonyms

ADIPOQ, apM-1, ADIPQTL1, Adipocyte, C1q and collagen domain-containing protein, Adipocyte complement-related 30 kDa protein

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis of NIH/3T3 using 21613-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis using 21613-1-AP
Human plasma was subjected to SDS PAGE followed by western blot with 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of 3T3-L1 using 21613-1-AP
3T3-L1 cells were subjected to SDS PAGE followed by western blot with 21613-1-AP (ADIPOQ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 10x lens).
IHC staining of mouse brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded rat brown adipose slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brown adipose using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 21613-1-AP (Adiponectin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human skin using 21613-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 21613-1-AP (ADIPOQ antibody) at dilution of 1:200 (under 10x lens).
IF Staining of NIH/3T3 using 21613-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Adiponectin antibody (21613-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Cat No. 21613-1-AP

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Tested Applications

Positive WB detected in	NIH/3T3 cells, 3T3-L1 cells, human plasma
Positive IHC detected in	human liver tissue, human skin tissue, human placenta tissue, human prostate cancer tissue, rat brown adipose, mouse skeletal muscle tissue, mouse brown adipose tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	NIH/3T3 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 20 publications below
IHC	See 1 publications below
IF	See 6 publications below

Product Information

21613-1-AP targets Adiponectin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Adiponectin fusion protein Ag16304
Full Name	adiponectin, C1Q and collagen domain containing
Calculated Molecular Weight	244 aa, 26 kDa
Observed Molecular Weight	29 kDa
GenBank Accession Number	BC096308
Gene Symbol	Adiponectin
Gene ID (NCBI)	9370
RRID	AB_2878891
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adiponectin (AdipoQ), an adipocyte-derived hormone, is one of the most abundant adipokines in the blood circulation. Adiponectin modulates a number of metabolic processes, including improving INS sensitivity and anti-inflammatory activity. The role of AdipoQ in reproduction is not yet fully understood, but the expression of AdipoQ in reproductive tissues has been observed in various animals and humans, including chicken testis, bovine ovary, and human placenta. Adiponectin exerts its effects by activating a range of different signaling molecules via binding to two transmembrane AdipoQ receptors, AdipoR1 and AdipoR2. AdipoR1 is expressed primarily in the skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. AdipoQ May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors.

Protocols

Product Specific Protocols
WB protocol for Adiponectin antibody 21613-1-AP	Download protocol
IHC protocol for Adiponectin antibody 21613-1-AP	Download protocol
IF protocol for Adiponectin antibody 21613-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IF	Diabetes PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ Authors - Yi Zhong View Article
human	WB	Int J Mol Sci Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells. Authors - Weiyun Zhang View Article
mouse	WB	Respir Res Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice Authors - Jong Huat Tee View Article
mouse	WB	J Nutr Biochem All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes Authors - Yuki Mori View Article
human	WB, IF	Sci China Life Sci Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin. Authors - Yue Wang View Article
mouse	WB	Dig Dis Sci Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation. Authors - Minghao Xie View Article

Adiponectin Polyclonal antibody

Adiponectin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis of NIH/3T3 using 21613-1-AP

WB analysis using 21613-1-AP

WB analysis of 3T3-L1 using 21613-1-AP

IHC staining of human liver using 21613-1-AP

IHC staining of mouse brown adipose using 21613-1-AP

IHC staining of human placenta using 21613-1-AP

IHC staining of human placenta using 21613-1-AP

IHC staining of human prostate cancer using 21613-1-AP

IHC staining of human prostate cancer using 21613-1-AP

IHC staining of rat brown adipose using 21613-1-AP

IHC staining of rat brown adipose using 21613-1-AP

IHC staining of mouse skeletal muscle using 21613-1-AP

IHC staining of mouse skeletal muscle using 21613-1-AP

IHC staining of mouse brown adipose using 21613-1-AP

IHC staining of human skin using 21613-1-AP

IHC staining of human skin using 21613-1-AP

IF Staining of NIH/3T3 using 21613-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Piperine Improves Lipid Dysregulation by Modulating Circadian Genes Bmal1 and Clock in HepG2 Cells.

Isthmin-1 attenuates allergic Asthma by stimulating adiponectin expression and alveolar macrophage efferocytosis in mice

All-trans retinoic acid induces lipophagy through the activation of the AMPK-Beclin1 signaling pathway and reduces Rubicon expression in adipocytes

Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.

Adiponectin Alleviates Intestinal Fibrosis by Enhancing AMP-Activated Protein Kinase Phosphorylation.