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ADHFE1 Polyclonal antibody

ADHFE1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14674-1-AP

Synonyms

ADH8, ADHFE1, HMFT2263, HOT

Validation Data Gallery

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  • Western Blot (WB) analysis of rat kidney tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    WB analysis of rat kidney using 14674-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ADHFE1 Polyclonal antibody (14674-1-AP)

    WB analysis using 14674-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14674-1-AP (ADHFE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human liver using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human liver using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon cancer using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ADHFE1 Polyclonal antibody (14674-1-AP)

    IHC staining of human colon cancer using 14674-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 14674-1-AP (ADHFE1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat kidney tissue, mouse kidney tissue, mouse liver tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14674-1-AP targets ADHFE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADHFE1 fusion protein Ag6382
Full Name alcohol dehydrogenase, iron containing, 1
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa, 40-45 kDa
GenBank Accession NumberBC064634
Gene Symbol ADHFE1
Gene ID (NCBI) 137872
RRIDAB_2221451
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ADHFE1 gene encodes hydroxyacid-oxoacid transhydrogenase which is responsible for the oxidation of 4-hydroxybutyrate in mammalian tissues. It belongs to the iron-containing alcohol dehydrogenase family. ADHFE1 has 4 isoforms produced by alternative splicing with the MW of 50, 45, 32, 27 kDa.

Protocols

Product Specific Protocols
WB protocol for ADHFE1 antibody 14674-1-APDownload protocol
IHC protocol for ADHFE1 antibody 14674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Circ Cardiovasc Genet

Proteome-Based Systems Biology Analysis of the Diabetic Mouse Aorta Reveals Major Changes in Fatty Acid Biosynthesis as Potential Hallmark in Diabetes Associated Vascular Disease.

Authors - Holger Husi
View Article