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ADH7 Polyclonal antibody

ADH7 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 23425-1-AP

Synonyms

ADH 4, ADH7, Gastric alcohol dehydrogenase, Retinol dehydrogenase

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ADH7 Polyclonal antibody (23425-1-AP)

    WB analysis of HeLa using 23425-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using ADH7 Polyclonal antibody (23425-1-AP)

    WB analysis of HEK-293 using 23425-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using ADH7 Polyclonal antibody (23425-1-AP)

    WB analysis of A549 using 23425-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 23425-1-AP (ADH7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ADH7 Polyclonal antibody (23425-1-AP)

    IHC staining of human liver using 23425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

  • Immunohistochemistry (IHC) staining of human liver tissue using ADH7 Polyclonal antibody (23425-1-AP)

    IHC staining of human liver using 23425-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver slide using 23425-1-AP (ADH7 Antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ADH7 Polyclonal antibody (23425-1-AP)

    IF Staining of HepG2 using 23425-1-AP

    Immunofluorescent analysis of HepG2 cells, using ADH7 antibody 23425-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23425-1-AP is ADH7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH7 fusion protein Ag19340
Full Name alcohol dehydrogenase 7 (class IV), mu or sigma polypeptide
Calculated Molecular Weight 386 aa, 41 kDa
Observed Molecular Weight 45-47 kDa
GenBank Accession NumberBC131512
Gene Symbol ADH7
Gene ID (NCBI) 131
RRIDAB_2879277
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ADH7(Alcohol dehydrogenase class 4 mu/sigma chain) is also named as gastric alcohol dehydrogenase, retinol dehydrogenase and belongs to the zinc-containing alcohol dehydrogenase family. The ubiquitous expression pattern of ADH7 during embryogenesis has led to the notion that the enzymatic conversion of retinol to retinal occurs more or less uniformly and plays no role in the spatial or temporal regulation of RA synthesis during embryonic development(PMID:17473173). It has 2 isoforms produced by alternative splicing and can exsit as a homodimer(PMID:18479436).

Protocols

Product Specific Protocols
WB protocol for ADH7 antibody 23425-1-APDownload protocol
IHC protocol for ADH7 antibody 23425-1-APDownload protocol
IF protocol for ADH7 antibody 23425-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Aging (Albany NY)

Mettl3-mediated m6A modification plays a role in lipid metabolism disorders and progressive liver damage in mice by regulating lipid metabolism-related gene expression

Authors - Guanqi Dai
View Article