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ADH6 Polyclonal antibody

ADH6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13288-2-AP

Synonyms

EC:1.1.1.1, ADH-5

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ADH6 Polyclonal antibody (13288-2-AP)

    WB analysis using 13288-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13288-2-AP (ADH6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using ADH6 Polyclonal antibody (13288-2-AP)

    WB analysis of human liver using 13288-2-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13288-2-AP (ADH6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using ADH6 Polyclonal antibody (13288-2-AP)

    IHC staining of mouse kidney using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using ADH6 Polyclonal antibody (13288-2-AP)

    IHC staining of mouse liver using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat kidney tissue using ADH6 Polyclonal antibody (13288-2-AP)

    IHC staining of rat kidney using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using ADH6 Polyclonal antibody (13288-2-AP)

    IHC staining of rat liver using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using ADH6 Polyclonal antibody (13288-2-AP)

    IHC staining of human liver using 13288-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13288-2-AP (ADH6 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using ADH6 Polyclonal antibody (13288-2-AP)

    IF Staining of MCF-7 using 13288-2-AP

    Immunofluorescent analysis of MCF-7 cells, using ADH6 antibody 13288-2-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, rat liver tissue
Positive IHC detected inhuman liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13288-2-AP targets ADH6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH6 fusion protein Ag3984
Full Name alcohol dehydrogenase 6 (class V)
Calculated Molecular Weight 368 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC039065
Gene Symbol ADH6
Gene ID (NCBI) 130
RRIDAB_2273710
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ADH6 antibody 13288-2-APDownload protocol
IHC protocol for ADH6 antibody 13288-2-APDownload protocol
IF protocol for ADH6 antibody 13288-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols