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ADH4 Polyclonal antibody

ADH4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16474-1-AP

Synonyms

ADH 2, ADH4, Alcohol dehydrogenase 4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using ADH4 Polyclonal antibody (16474-1-AP)

    WB analysis of HepG2 using 16474-1-AP

    WB result of ADH4 antibody (16474-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ADH4 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using ADH4 Polyclonal antibody (16474-1-AP)

    WB analysis using 16474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using ADH4 Polyclonal antibody (16474-1-AP)

    WB analysis of mouse brain using 16474-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ADH4 Polyclonal antibody (16474-1-AP)

    WB analysis of HeLa using 16474-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16474-1-AP (ADH4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using ADH4 Polyclonal antibody (16474-1-AP)

    IHC staining of human kidney using 16474-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using ADH4 Polyclonal antibody (16474-1-AP)

    IHC staining of human kidney using 16474-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16474-1-AP (ADH4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ADH4 Polyclonal antibody (16474-1-AP)

    IHC staining of human heart using 16474-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using ADH4 Polyclonal antibody (16474-1-AP)

    IHC staining of human heart using 16474-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16474-1-AP (ADH4 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, HeLa cells, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 3 publications below

Product Information

16474-1-AP targets ADH4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ADH4 fusion protein Ag9568
Full Name alcohol dehydrogenase 4 (class II), pi polypeptide
Calculated Molecular Weight 380 aa, 40 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC022319
Gene Symbol ADH4
Gene ID (NCBI) 127
RRIDAB_2223855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

This gene encodes the class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. ADH4 plays a critical role in alcoholism development and alcohol toxicology and is also associated with the development of esophageal cancer(PMID:33556337, 26039424, 24051444, 22147505).

Protocols

Product Specific Protocols
WB protocol for ADH4 antibody 16474-1-APDownload protocol
IHC protocol for ADH4 antibody 16474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Immunol

Identification and validation of a tyrosine metabolism-related prognostic prediction model and characterization of the tumor microenvironment infiltration in hepatocellular carcinoma

Authors - Yangying Zhou
View Article
humanIHC

Front Oncol

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Authors - Tingbo Ye
View Article
humanIHC

BMC Cancer

ADH4-a potential prognostic marker for hepatocellular carcinoma with possible immune-related implications

Authors - Ling Li
View Article